Description

 Dyne RNA LoadingSTAR replace toxic Ethidium Bromide (EtBr), a potent mutagen, for the visualization of RNA in agarose and polyacrylamide gel electrophoresis. 

Dyne RNA LoadingSTAR contains red color tracking dye and a safe, non-carcinogen red fluorescent dye. 

Red dye is used for monitoring the agarose gel while it is running (approx 1 kb). During the gel electrophoresis, the agarose gel will reveal a red band. 

Thus, the gel can be directly visualized under UV light without any extra staining steps. 

In addition, RNA stained with the red fluorescent dye will emit a red light (595 nm) similar to Ethidium Bromide (EtBr).

Protocol

1. Mix your samples with the Dyne RNA LoadingSTAR in a 1:5 ratio. For example, add 1μl of the Dyne RNA 

LoadingSTAR to 5 μl of RNA sample; or 2 μl of the Dyne RNA LoadingSTAR to 10 μl of RNA sample. 

2. Run the agarose gel and visualize it under LED or UV light when ready. Take a picture as needed commonly

Components

● red dye, non-carcinogen red fluorescent dye